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. 2013 Dec;87(23):12967–12979. doi: 10.1128/JVI.02080-13

Fig 6.

Fig 6

The σ1s N-terminal basic cluster is not required for reovirus replication in cell culture. (A) L929 cells were infected with rsT3D, rsT3D σ1s R14L, rsT3D σ1s R14L/R15L, or rsT3D σ1s R14L/R15L/R19L at an MOI of 1 PFU/cell. Titers of virus in cell lysates were determined by plaque assay at 24 and 48 h postinfection. Results are expressed as the mean viral yields from triplicate samples. Error bars indicate SD. (B) L929 cells were mock infected or infected with rsT3D, rsT3D σ1s R14L, rsT3D σ1s R14L/R15L, or rsT3D σ1s R14L/R15L/R19L at an MOI of 100 PFU/cell. Whole-cell lysates were prepared from infected cells at 48 h postinfection and resolved by SDS-polyacrylamide gel electrophoresis. Reovirus proteins were detected by immunoblotting using a reovirus-specific polyclonal antiserum. Reovirus proteins are labeled on the right.