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Mutations in the σ1s amino-terminal basic cluster do not affect nuclear translocation of the protein. Cos-7 cells were either mock infected or infected with rsT3D, rsT3D σ1s R14L, rsT3D σ1s R14L/R15L, or rsT3D σ1s R14L/R15L/R19L at an MOI of 10 PFU/cell. Following incubation for 24 h, cells were fixed and stained with a T3D σ1s-specific monoclonal antibody or a reovirus-specific polyclonal antiserum. Nuclei were stained with DAPI.
