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Interaction between IE1, WSV056, and Drosophila RBF1. HA-RBF1 was coexpressed in High Five cells with FLAG-IE1, FLAG-IE1C216G, FLAG-WSV056, or FLAG-WSV056C217G, separately. The pIEx-4 vector was used as a negative control. Cells were lysed, the FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2 affinity gel, and then the immunoprecipitated complexes were analyzed by Western blotting with anti-HA antibody and anti-FLAG antibody.
