Table 1.
Primers used in this study
| Primer | Sequence (5′–3′) |
|---|---|
| RBL-HA forward | TGAAGCTTTACCCATACGATGTTCCAGATTACGCTATGAGTGACACTGATCTAGGCTC |
| RBL-HA reverse | ATGCTCGAGCTAAGGTTGCTCTGGTGGGA |
| WSV056 C217G-FLAG forward | ATGGGATCCATGGACTACAAGGATGACGATGACAAGG |
| WSV056 C217G-FLAG reverse | ATGGGATCCTTATTGTACCAAAAACTCAGAAATCTCATCTCCTGA |
| IE1 C217G-FLAG forward | ATGGGATCCATGGACTACAAGGATGACGATGACAA |
| IE1 C216G-FLAG reverse | ATGGGATCCTTATACAAAGAATCCAGAAATCTCATCTCCTGT |
| hRluc forward | ATGGGATCCGCTTCCAAGGTGTACGACCC |
| hRluc reverse | ATGCTCGAGCTGCTCGTTCTTCAGCACGC |
| EGFP forward | ATGAAGCTTGTGAGCAAGGGCGAGGA |
| EGFP reverse | ATGCTCGAGTTACTTGTACAGCTCGTCCATG |
| WSV056-EGFP forward | ATGGGATCCCGCCTCAGTCTTTGAAGACCC |
| WSV056-EGFP reverse | ATGGGTACCTTGTACCAAAAACTCAGAAATCTC |
| IE1-EGFP forward | ATGGGATCCCGCCTTTAATTTTGAAGACTCTACA |
| IE1-EGFP reverse | ATGGGTACCTACAAAGAATCCAGAAATCTCATC |
| WSV056 C217G-EGFP forward | ATGGGATCCCGCCTCAGTCTTTGAAGACCC |
| WSV056 C217G-EGFP reverse | ATGGGTACCTTGTACCAAAAACTCAGAAATCTC |
| IE1 C216G-EGFP forward | ATGGGATCCC GCCTTTAATTTTGAAGACTCTACA |
| IE1 C216G-EGFP reverse | ATGGGTACCTACAAAGAATCCAGAAATCTCATC |
| orf-1 forward | AATTACGAGAACATCCAGCTGA |
| orf-1 reverse | GAATCTTTCTGAGATCGTTGAA |
| orf-2 forward | GAATTGATGCGAGACCGTCA |
| orf-2 reverse | TGTGGCAGAGAGCCTATCCC |
| middle forward | ATTTGCCAAGTTAAGATTACAAA |
| middle reverse | GCTGGTTCCTATAGTGCTTCATA |
| 5′-race primer | ACAGCTGACAAGATCCTGAGGAATACAT |
| 3′-race primer | TCTTTCTCCATTGCCAGTAGTACGTCAC |