Fig 3.
Rates of cell fusion for gD V231W in the presence and absence of HSV receptor nectin-1. Rluc8 plasmids were used to measure fusion rates. (A) Target cells consisted of C10 cells transfected with Rluc81-7. (B) Target cells consisted of B78H1 cells (no receptor) plus Rluc81-7. In both experiments, effector cells contained plasmids for Rluc88-11 plus plasmids for gB, gH/gL, and either WT gD, gD V231W, or empty vector. The experiments whose results are presented in panels A and B were done at different times. Target cells were overlaid onto receptor cells that had been preincubated with the EnduRen substrate for 1 h. Luminescence readings were taken at the times indicated on the x axis. The experiments whose results are presented in panels A and B were done independently.