Fig 4.

Reactivity of gD V231W with conformationally sensitive antibodies. (A) Native Western blotting of gD 306t or V231W with MAb epitopes that are near the receptor binding face of gD (DL11, MC23, AP7) or that map to the opposite side of gD proximal to where the C terminus attaches to the Ig-like core of gD (MC5, A18). Asterisk, full-length gD. (B) gD was adhered to an ELISA plate for MAb binding. Mapping of epitopes to the receptor binding region of gD indicated structural changes associated with V231W, while subtle changes were observed in epitopes not associated with the receptor binding face of gD.