Fig 6.

Biosensor binding of gD to HSV receptor HVEM or nectin-1. HVEM 200t was captured and then bound to gD 306t (A) or gD V231W (B). Nectin-1 245t was captured and bound to gD 306t (C) or gD V231W (D). gD concentrations of 0 μM, 1 μM, 2 μM, 4 μM, 6 μM, 8 μM, and 10 μM were injected in sequential cycles, with the receptor surface being regenerated between cycles. Curves were fit using a 1:1 Langmuir binding model, yielding the binding constants described in Table 1. RU, resonance units.