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. 2013 Nov;33(22):4360–4370. doi: 10.1128/MCB.00870-13

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Fig 2 — Uaf1^+/− MEFs are hypersensitive to DNA-damaging agents. (A) Immunoblots of the lysates from primary Uaf1^+/− and Uaf1^+/+ MEFs. Usp1 antibody against the C-terminal epitope of Usp1 was used for immunoblots. (B to F) Survival plots of Uaf1^+/− and Uaf1^+/+ MEFs exposed to various DNA-damaging agents. The MEFs were exposed to MMC, UV, IR, etoposide, or AZD 2281, and survival was determined using a colony assay. P < 0.005 for Uaf1^+/− versus Uaf1^+/+ MEFs. (G to I) The Uaf1^+/− and Uaf1^+/+ MEFs were exposed to MMC for 48 h, and metaphase spreads of chromosomes were scored for chromosomal abnormalities. (G and H) Aberrations per cell (G) and percentages of cells with radials (H). (I) Representative images of metaphase spreads of the chromosomes. The arrows indicate radials in Uaf1^+/− MEFs upon MMC exposure. The data in panels B to H are representative of two independent experiments in triplicate, and means ± standard errors are shown.