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. Author manuscript; available in PMC: 2014 Aug 20.
Published in final edited form as: Circulation. 2013 Jul 3;128(8):10.1161/CIRCULATIONAHA.113.003602. doi: 10.1161/CIRCULATIONAHA.113.003602

Figure 2.

Figure 2

Proton pump inhibitors (PPIs) inhibit DDAH activity. A) Colorimetric assay showing reduced production of L-citrulline from ADMA. B) Fluorimetric assay showing inhibited signal associated with DDAH enzymatic activity. In A) L-citrulline conc. was calculated from standard curve. In B) ebselen was used as a positive assay control 27. Data is from triplicate experiments (Mean ± SEM) at 50 μM final compound concentration. *p<0.05 when the PPIs are compared to the vehicle control by One-Way ANOVA followed by Bonferroni posttest correction.