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The PPI omeprazole reduces the expression of total and active endothelial NOS. Regulation of total endothelial NOS (eNOS) and active eNOS (phospho-eNOS) by omeprazole was studied by Western blot using endothelial cell (EC) lysate exposed to omeprazole or vehicle. ECs were treated with VEGF (50 ng/mL) as positive eNOS phosphorylation control. The expression of each protein was normalized to β-actin (ACTB).
