Fig 1.

CDC25B is a pro-influenza A virus host factor. To evaluate the role of CDC25B during IAV infection, A549 cells were transfected with nontargeting siRNA (siNEG) or siRNA targeting CDC25B (siCDC25B). (A and B) At 48 h posttransfection, cells were infected with influenza A/WSN/33 virus at MOI = 0.05 and fixed (A) or harvested (B) for RNA isolation at 48 hpi. (A) Following fixation, cells were stained for influenza virus NP and nuclei (DAPI). Cells were visualized using a high-content imaging system. Three representative images for each condition are shown (top), and percentages of NP-positive cells were quantified from 10 fields (bottom). (B) Culture supernatants from infected cells were used for virus titration in MDCK cells. (C) Knockdown of CDC25B mRNA expression was verified using qRT-PCR normalized to GAPDH. *, P < 0.05; **, P < 0.01.