Fig 3.

CDC25B inhibitor NSC95397 limits influenza A and B infections in vitro. (A to D) BEAS2B cells were infected with influenza A/WSN/33 virus (A and B) or B/Florida/04/06 virus (C and D) at MOI = 0.05 in the presence of increasing NSC95397 concentrations (in 1/4-log increments). At 24 hpi, cell supernatants were harvested for virus titration in MDCK cells. Dotted lines indicate 50% inhibition of virus titer (IC₅₀). TCID₅₀, 50% tissue culture infective dose. (E) BEAS2B cells were treated with increasing doses of NSC95397 (in 1/2-log increments), and cellular cytotoxicity was assessed at 24 h posttreatment using a ToxiLight bioassay kit. Percent cell viability of NSC95397-treated cells was determined relative to nontreated cells (100% viability) and lysed cells (0% viability). The dotted line indicates 50% cytotoxicity (CC₅₀). (D) IC₅₀ and CC₅₀ values were determined using the nonlinear regression method. Selectivity indices were calculated as the ratio of CC₅₀ to IC₅₀.