Fig 7.
Effect of removal of the C-terminal 32 amino acids of B2 on viral replication in DL-1 and BHK21 cells and in Drosophila flies. (A) Expression analysis of R1B21-74 in DL-1 cells at 24 h posttransfection. Shown is an image of an EtBr-stained gel on which total RNA extracts from cells transfected with the indicated constructs were loaded (top panel), as well as immunoblots for the detection of coat protein and B2 in corresponding cell lysates (lower panels). R1KOR3 refers to the RNA1 construct in which synthesis of RNA3 was knocked out. (B) Analogous analysis for the expression of R1B21-74 in BHK21 cells. (C) Quantification of viral RNA2 in flies 4 days postinfection with wt or B21-74 mutant FHV particles (1,000 PFU/fly). Data are normalized relative to RpL32 and represent the means and standard deviations of results from three independent experiments. The P value from Student's t test is indicated. (D) Survival curves for adult flies (10 per group) injected with wt or B21-74 mutant FHV particles (1,000 PFU/fly). Data represent the means and standard deviations from 3 independent groups. **, P < 0.005 by log-rank (Mantel-Cox) test.