Fig 3.
A soluble form of HSV gH/gL (gHt/gL) suffices to induce NF-κB activation and IFNB1 production. (A to C) sh-β3 and control (Ctrl) cells, transfected with NF-κB–luc and Renilla luc, as described in the legend to Fig. 2, were exposed to gHt/gL (1.5 μM) for 4 h or infected with R7910 (20 PFU/cell). The production and characterization of gHt/gL were described previously (32). Where indicated, gHt/gL was preincubated for 1 h with MAb 52S (33) to gH/gL (gHt/gL + 52S) or heat inactivated for 15 min at 100°C (H-gHt/gL). Each column represents the average of triplicates ± standard deviation. (D to F) sh-β3 and control cells were exposed to gHt/gL (1.5 μM) for 6 h, infected with R7910 (20 PFU/cell), or left uninfected. IFNB1 mRNA levels were determined as detailed in the legend to Fig. 2, 6 h after infection. The mRNA level is expressed as fold increase in infected cells relative to that of uninfected cells (set as 1). Each column represents the average of triplicates ± standard deviation.