Fig 3.

Flotation analysis of RSV and HIV-1 MA multimers. Liposomes were made with the following compositions: PC/PS (70/30), PC/PS (40/60), PC/PS/cholesterol (Chol) (34/30/36), and PC/PS/PI(4,5)P2 (68/30/2). All proteins were radiolabeled in reticulocyte translation reactions. (A) Representative flotation results of MA multimers to PC/PS/Chol liposomes. Symbols denoting multimeric state are the same as for Fig. 2. The radioactive protein was scored either as membrane bound (MB) or as non-membrane bound (NMB). (B and C) Percentages of total protein found associated with liposomes for RSV (B) and HIV-1 (C) MA multimers. Error bars represent standard deviations from the means. P values for Student's t test for comparison of MA-FKBP monomer to MA-FKBP dimer, MA-Ccmk4 hexamer, and Gag (*, P < 0.05; **, P < 0.005).