Fig 3.

Treatment with AS1411 blocks interaction between NCL and DENV C and affects colocalization. (A) Co-IP of HEK293 cells transfected with expression vector GFP-DVC. The cells were treated with AS1411 or negative-control CRO (10 μM). Co-IP was performed as previously described, and the Western blots were stained with antibodies to NCL or GFP. (B) Confocal microscopy of HEK293 cells left uninfected or infected with DENV, followed by no treatment or treatment with AS1411 or CRO (10 μM). Samples were examined for localization of DENV C (green) and NCL (red). The nucleus was stained with DAPI (blue). Colocalization of DENV C and NCL is shown in white. (C) Colocalization coefficients of DENV C and NCL in uninfected cells or cells infected with DENV and left untreated or treated with AS1411 or CRO (10 μM), as determined by Pearson's linear correlation coefficient. Significance was determined using a P value of 0.01 (indicated by an asterisk [*]) from 10 images collected from two independent experiments. ns, not significant. The error bars indicate SD.