Table 2.
Comparison of HN-H interaction residues of PIV5 F and measles virus Fa
| Serial no. | Mutated PIV5 F residue | Corresponding measles virus F residue | Location in PIV5 F prefusion structure | Possible PIV5 HN-interacting residue | Corresponding measles virus H-interacting residue |
|---|---|---|---|---|---|
| 1 | A392 | T402 | Domain II (Ig-like) | Y | ND |
| 2 | V393 | I403 | Domain II (Ig-like) | Y | ND |
| 3 | I394 | I404 | Domain II (Ig-like) | ND | |
| 4 | L395 | N405 | Domain II (Ig-like) | Y | ND |
| 5 | Q396 | Q406 | Domain II (Ig-like) | ND | |
| 6 | S382 | S392 | Domain II (Ig-like) | Y | ND |
| 7 | L384 | L394 | Domain II (Ig-like) | Y | Y |
| 8 | N298 | S308 | Domain I (h.p.) | Y | ND |
| 9 | F295 | N305 | Domain I (h.p.) | ND | |
| 10 | R368 | R378 | Domain I (h.p.) | ND | |
| 11 | T312 | Q322 | Domain I (h.p.) | N | Y |
| 12 | S314 | Y324 | Domain I (h.p.) | N | ND |
| 13 | Q339 | Y349 | Domain I (h.p.) | N | Y |
| 14 | V340 | P350 | Domain I | N | ND |
| 15 | V362 | S372 | Domain I | N | ND |
| 16 | S364 | S374 | Domain I | N | ND |
a
List of point mutants generated in PIV5 F and the corresponding residues of measles virus F as shown by sequence alignment. The residues in PIV5 F or measles virus F (49) that are implicated in interactions with HN or H are indicated. PIV5 F residues shown in gray do not reach the surfaces of cells. Y, yes (possible interaction); N, no (possibly not involved in direct interaction); h.p., hydrophobic pocket adjacent to the Ig-like domain (Ig-like); ND, not determined.