Skip to main content
. 2013 Dec;87(24):13741–13750. doi: 10.1128/JVI.02582-13

Fig 2.

Fig 2

Modulation of NFX1-123 expression in keratinocytes. (A) NFX1-123 mRNA expression by qPCR in 16E6 HFKs transduced with overexpressed wild-type FLAG-tagged NFX1-123 (FNFX1-123WT), isogenic scramble short hairpin RNA control (scr), short hairpin 1 RNA to NFX1-123 (sh1), or short hairpin 2 RNA to NFX1-123 (sh2). Relative levels of NFX1-123 mRNA were normalized to the mRNA levels of housekeeping gene 36B4. Values shown were the mean fold change in each sample compared to the scr vector control. Error bars represent 95% confidence intervals from the technical replicates shown (n = 3). (B) Representative immunoblot showing NFX1-123 protein expression in 16E6 HFKs. Cells were transduced as described for panel A, and densitometry was calculated using Quantity One. GAPDH is shown as a loading control. (C) NFX1-123 mRNA levels by qPCR of HFKs transduced with FNFX1-123WT, scr, sh1, or sh2. Relative levels of NFX1-123 mRNA were normalized to 36B4 mRNA levels. Values shown were the mean fold changes in each sample compared to the scr vector control. Error bars represent 95% confidence intervals from the technical replicates shown (n = 3). (D) Representative immunoblot showing NFX1-123 protein expression in HFKs. Cells were transduced as described for panel C, and densitometry was calculated using Quantity One. GAPDH is shown as a loading control.