Fig 6.

PRC2 rapidly targets HCMV chromatin for H3K27me3 during infection of pluripotent NT2D1 cells. NT2D1 cells were infected with AD169 (A) or TB40E (B) at an MOI of 1.0. At 48 hpi, ChIP was performed using anti-H3K27me3, anti-pan-H3, and normal rabbit IgG. The material recovered was analyzed by qPCR using primer-probe sets specific for the indicated loci (abbreviations explained in the legend to Fig. 5A). Each bar represents the IgG-normalized H3K27me3/H3 ratio for the viral locus queried, the negative-control region, PLCB4, or the positive-control region, HOXA9. Ratios are means of data collected from at least three biological replicates; error bars represent standard errors of the means. Asterisks indicate P values of <0.05 (*), <0.01 (**), <0.001 (***), or <0.0001 (****) by paired, two-tailed t tests.