Fig 7.

Treatment with the EZH2-specific inhibitor GSK343 reactivates the AD169 lytic transcription program in THP1 monocytes. (A) Schematic diagram of the treatment and infection protocol. Cells were infected with AD169 or TB40E at an MOI of 1.0; infected cells were treated daily with either DMSO, 5 μM DZnep, or 10 μM GSK343. (B) HCMV lytic mRNA expression. qPCR was performed, and data were analyzed and presented, as described above. Significance was calculated using paired, two-tailed t tests. Asterisks indicate P values of <0.05 (*), <0.01 (**), or <0.0001 (****). (C) ChIPs were performed using anti-H3K27me3, anti-pan-H3, and normal rabbit IgG. The material recovered was analyzed by qPCR using primer-probe sets specific for the indicated cellular loci, before and after GSK343 treatment. Each bar represents the IgG-normalized H3K27me3/H3 ratio for the locus indicated.