Table 1.

Primer and probe sequences used in this study

Target	HCMV straina	Supplier	Sequenceb
IE1/2	AD169	IDT	Fwd, TGCAATCCTCGGTCACTTG
			Rev, GACCCTGATAATCCTGACGAG
			Probe, CTAAGGCCACGGAGCGAGACATC
IE1/2	TB40E	IDT	Fwd, AGAAAGATGGACCCTGACAAC
			Rev, AACATAGTCTGCAGGAACGTC
			Probe, CCCGAGACACCCGTGACCAAG
UL54	AD169/TB40E	IDT	Fwd, CTGGCTAAAATTCCGTTGCG
			Rev, GTCGTACCTTTGCTGTAGTGG
			Probe, TTGGGCAGGATAAAATCGCGGC
UL99	AD169/TB40E	IDT	Fwd, GGAAGTCGGAGGGATGTTG
			Rev, GGTGAGCCCCTGAAAGATG
			Probe, TCGTAGGAGCGTAGAGACACCTGG
MIE TSS	AD169	ABI	Fwd, CCGGTGTCTTCTATGGAGGT
			Rev, GCAGAGCTCGTTTAGTGAACC
			Probe, GCGTCTCCAAGGCGATCTGAC
MIE TSS	TB40E	IDT	Fwd, ACGCTGTTTTGACCTCCATAG
			Rev, GCGGTACTTACGTCACTCTTG
			Probe, CGGTGCATTGGAACGCGGATT
MIE enhancer	AD169	ABI	Fwd, CAAGTGGGCAGTTTACCGT
			Rev, TGTTCCCATAGTAACGCCAA
			Probe, ACTCCACCATTGACGTCAA
MIE enhancer	TB40E	IDT	Fwd, TTGGGCATACGCGATATCTG
			Rev, GCCTCATATCGTCTGTCACC
			Probe, ACTTTGGCGACTTGGGCGATTC
MIE exon 1	AD169	ABI	Fwd, AGACCCATGGAAAGGAACAG
			Rev, GGTGGAGGGCAGTGTAGTCT
			Probe, CGGCAGCAACGAGTACTGCT
MIE exon 4	TB40E	IDT	Fwd, CCCTTCACGATTCCCAGTATG
			Rev, CACCTCACTCTTCACCTCATG
			Probe, TGGCCTCATCTAACACCTGGTCC
UL69	AD169/TB40E	IDT	Fwd, TCGGTGGGATGAATTTGGTC
			Rev, CATGATAGCGTACTGTCCCTTC
			Probe, CGTGGTTGCTGGCGGTTGTCG
β2-Microglobulin	NA	IDT	Fwd, TGGATGACGTGAGTAAACCTG
			Rev, GGCATTCCTGAAGCTGACAG
			Probe, CTAAGGCCACGGAGCGAGACATC
HoxA9	NA	ABI	Fwd, ATTGTTTCAGAAGCCACACAGGCTG
			Rev, GGGAGCTCGCCAACCAAACACA
			Probe, TCGCCGTGGCTCCCAGCT
PCLB4	NA	IDT	Fwd, ATAAACCCATACAGCGCCTAC
			Rev, ACTTAATCTCTGTGGCTCTGTG
			Probe, ACTGCCTCTTTTTATTTCGCGGGTCT
MYT1	NA	IDT	Fwd, TCACCCCAAAGGCAAGTATC
			Rev, ATCCTCAGTTTGACCAGTGC
			Probe, TGAGCACAAGGACATAAATGCAAGCC

^aNA, not applicable.

^bFwd, forward primer; Rev, reverse primer.