Fig 2.
The interaction of rhesus BST-2 with Vpu involves their cytoplasmic domains. (A) Schematic representation of the BST-2 variants used in this analysis. The cartoon is not drawn to scale (the KGC tag and human BST-2 comprise 51 and 180 residues, respectively). (B) The physical interaction between NL4-3 Vpu or VpuRD and rhBST-2 variants was quantified by flow cytometry as described for Fig. 1B. Error bars reflect the standard errors of the means from three independent experiments. ***, P < 0.01; **, 0.01 < P < 0.05. The expression of KGC-tagged BST-2 variants and KGN-tagged Vpu mutants was assessed by immunoblotting as described for Fig. 1B. The KGN fragment encoded by the “tag-only” vector in lanes 1, 2, and 3 has a faster mobility and is not visible here.