Fig 5.

MV HRC peptides inhibit MV infection of organotypic brain explants. Hippocampal slices from SLAM TG murine brains were treated with the indicated concentrations of either HRC2 or HRC4 for 5 h or left untreated (control) and infected with MV-IC323-EGFP at 10⁴ PFU/slice for 3 days. (A) Cultures from MV-infected explants (green) were observed by immunofluorescence microscopy. (B) Total RNA was harvested from organotypic slices at 3 days postinfection, and the level of MV N gene expression was quantified by reverse transcription-qPCR. Results are expressed as means ± standard deviations of triplicate cultures (**, P < 0.01; ***, P < 0.001 [unpaired t test]).