Fig 1.

Internalization assay with VP1u-containing and VP1u-lacking B19V capsids. (A) Schematic depiction of the B19V capsid and the VP1u region. In red is the truncated region (ΔN29). In green is the epitope recognized by the N-VP1u MAb, and in blue is the epitope recognized by the PLA₂ Ab. (B) Internalization assay with different B19V capsids. Following binding at 4°C, UT7/Epo cells were incubated at 37°C for 30 min. Cells were trypsinized and washed to remove uninternalized particles and fixed for IF. Internalized capsids were detected with Ab 860-55D against capsids.