Fig 4.

A proportion of MCPyV ST colocalizes with NEMO. Following transfection of Huh7 (A) and MCC13 (B) cells with either pEGFP or pEGFP-ST in the presence of pNEMO-myc, after 24 h, cells were fixed and permeabilized, and GFP fluorescence was analyzed by direct visualization, whereas NEMO was identified by indirect immunofluorescence using a Myc-specific antibody. (C) A similar experiment was repeated, but Huh7 cells were stained with LC3- and Myc-specific antibodies. (D) 293 cells were transfected with pEGFP-cI or pEGFP-ST. After 12 h, the appropriate wells were either mock treated or treated with either a protease inhibitor (PI) cocktail or 3-methyladenine for 12 h. Cell lysates were then analyzed by immunoblotting using the indicated antibodies. DAPI, 4′,6-diamidino-2-phenylindole.