Fig 5.

MCPyV ST inhibits phosphorylation of IκB and prevents nuclear translocation of the NF-κB heterodimer. (A) i293-ST cells remained uninduced (Un) or induced (In) for MCPyV ST expression. Cells were then serum starved before TNF-α was used to stimulate activation of the NF-κB pathway. Nuclear and cytoplasmic fractions were then isolated and analyzed by immunoblotting to detect phosphorylation of IκB and levels of nuclear translocation of p65 and p50 using the indicated antibodies. (B) Densitometry quantification of the Western blots was carried out using ImageJ software and is shown as a percentage of relative densitometry normalized to the loading control GAPDH. Standard deviations of 3 replicated experiments are shown.