Table 1.

Host range of phage ΦAA91-ss in a collection of cdt-negative strains^a

Host	Strain or serotype	Originb	stx variant	Lysis	Transduction
E. coli	C600	ATCC 23724		−	−
E. coli	DH5α	Invitrogen		−	−
E. coli	O157:H7	ATCC 43888		++	−
E. coli	WG5	ATCC 700078		++	+
Salmonella Typhimurium	WG49	ATCC 700730		−	−
S. sonnei	866	Clinical		++	+
S. sonnei	30673	Clinical		+	−
S. sonnei	30674	Clinical		++	+
S. sonnei	30676	Clinical		++	+
S. sonnei	30679	Clinical		++	+
S. sonnei	30682	Clinical		++	+
S. flexneri	805-F	Clinical		+	−
E. coli	O111	Clinical		+	−
E. coli	O26	Clinical		−	−
E. coli	O26	Clinical		−	−
E. coli	O157:H7	Clinical	stx2a, stx2c	−	−
E. coli	O4:H4	Clinical	stx2a	++	−
E. coli	O15:H16	Clinical	stx2a	−	−
E. coli	O174:H-	Clinical	stx2c	++	−
E. coli	O104:H4	Clinical	stx2a	−	−
E. coli	O146:H21	H	stx2d	−	−
E. coli	O90:H-	M	stx2d	−	−
E. coli	O171:H2	C	stx2c	−	−
E. coli	O171:H2	C	stx2c	−	−
E. coli	O181:H49	C	stx2a	++	−
E. coli	O181:H20	C	stx2c	+	−
E. coli	O2:H25	C	stx2g	+	−
E. coli	O136:H1	C	stx2g	+	+
E. coli	O90:H-	H	stx2d	−	−
E. coli	ONT:H2	C	stx2c, stx2d	+	−
E. coli	O2:H25	C	stx2g	−	−
E. coli	O1:H20	C	stx2a	+	−
E. coli	O8:H31	C	stx2g	−	−
E. coli	O1:H20	C	stx2a	+	−
E. coli	O157:H-	C	stx2c	+	−
E. coli	O22:H8	C	stx2c	+	+
E. coli	O166:H21	H	stx2c	−	−
E. coli	O157:H-	C	stx2c	−	−
E. coli	O156:H8	C	stx2c	+	+
E. coli	O1:H20	C	stx2a	−	−
E. coli	O171:H2	C	stx2a, stx2c	+	−
E. coli	O76:H2	C	stx2c	++	−
E. coli	O113:H21	C	stx2a	+	+
E. coli	O100:H-	P	stx2e	+	−
E. coli	O171:H2	C	stx2c	−	−
E. coli	O28:H28	C	stx2a	+	+
E. coli	O8:H9	H	stx2e	−	−
E. coli	O181:H49	C	stx2a	+	−
E. coli	O171:H2	C	stx2c	−	−
E. coli	ONT:H-	C	stx2c	+	−
E. coli	O2:H25	M	stx2g	+	−
E. coli	ONT:H-	M	stx2e	−	−
E. coli	O22:H8	C	stx2c	+	+
E. coli	O157:H7	C	stx2c	+	−
E. coli	O157:H7	C	stx2c	+	−
E. coli	O22:H8	H	stx2c	++	+
E. coli	O1:H20	H	stx2a	−	−

^aLytic infection ability was determined by observation of lysis visualized directly on the plate and after hybridization with the cdtB probe: +, weak lytic spot; ++, strong lytic spot; −, negative. Transduction ability was evaluated by PCR on colonies obtained after infection with the Cdt phage.

^bH, strain isolated form raw urban wastewater (human fecal pollution); C, strain isolated from cattle wastewater; P, strain isolated from poultry wastewater; M, strain isolated from mixed animal wastewater.