Fig 1.

Ligand insertion sites on VSV-G. (A) Linear map of the VSV genome with GFP insertion. (B) Amino acid sequence of VSV-G (crystal structure), showing cRGD insertion sites. (C) Insertion site of ligands (bold letters) at the N terminus of the G protein. Only the signal peptide (italics) and a few residues of G are shown (underlined). *, an additional lysine residue was included to maintain the proper G sequence. (D) Crystal structure of the VSV G protein, showing cyclic RGD insertion sites. The Pymol program was used to edit the G protein crystal structure (PDB 2J6J).