Fig 1.

Us11 inhibits PACT-induced activation of RIG-I. (A) Influence on IFN-β promoter. HEK293 cells were transfected with pIFN-β-Luc reporter and expression plasmids for Flag-PACT, Flag-RIG-I, and Us11. Dual-luciferase assays were performed at 33 h posttransfection. Results represent the means ± standard deviations of three independent measurements of firefly luciferase activity normalized to Renilla luciferase activity. Differences between the selected groups were statistically assessed by a two-tailed Student's t test: p1 = 0.002; p2 = 0.087. (B) Influence on IRF3-binding elements (p1 = 0.012; p2 = 0.083). (C) Influence on κB enhancer (p1 = 0.416; p2 = 0.393).