Fig 7.

Efficiency of heat-induced ISVP-to-ISVP* transition is restored in viruses containing second-site reversions. ISVPs of viruses with second-site reversions alone or in combination in E89A (A), E89Q (B), or wild-type (C) μ1 backgrounds were generated by digestion of virions at 28°C. The ISVPs were heated at the indicated temperatures (°C) for 20 min and treated with trypsin at 4°C for 30 min. Samples were resolved by SDS-PAGE and stained using Coomassie brilliant blue. The positions of reovirus capsid proteins are shown. μ1δ resolves as δ (34). ϕ is too small to resolve on the gel.