Fig 4.

Release and multistep replication of HSV-1ΔVhs is inhibited in a tetherin-dependent manner. Caco-2 cells and Caco-2–tetherin shRNA cells were infected with HSV-1wt or HSV-1ΔVhs. (A) Infectious-HSV-1 titers both in supernatant (dark bars) and associated with cells (light bars) were established 16 h postinfection. HSV-1 release was calculated as percent of total infectious virus for each sample. Error bars represent standard errors of the means. ***, P < 0.005; **, P < 0.05; *, P < 0.1; n.s., P > 0.1. (B) Caco-2 cells (solid lines) and Caco-2–tetherin shRNA cells (dashed lines) were infected at a low MOI (0.01 PFU/cell) with HSV-1wt and HSV-1ΔVhs. Total infectious-virus yields at the indicated time points were determined by plaque assay on Vero cells. Error bars represent standard errors of the means for triplicate samples.