Fig 9.

Conservation of the bidirectional ORF73 LANA and K14/RF14 promoter structure of KSHV and RFHVMn. (A and B) Diagrammatic rendering (A) and sequence alignment (B) of the promoter region of the KSHV LANA/K14 genes (NC_009333) and the RFHV LANA/RF14 genes, showing the initiating codons and the positions of the conserved splice donor (SD) and splice acceptor (SA) sites generating the latent spliced LANA transcript from the constitutive promoter (LANApc). The Rta-inducible LANApi promoter of KSHV LANA is indicated, with its associated RBP-Jκ binding sites and Rta response element (RRE) located within the intron region spliced out of the LANApc transcript (see the text). The position of a putative LANApi promoter for RFHV LANA is shown. (C) Alignment of the RBP-Jκ binding motifs of KSHV and RFHV indicated in panels A and B. Nucleotide numbers are based on the LANA translational initiation codon.