. 2013 Dec;87(24):13676–13693. doi: 10.1128/JVI.02331-13

Table 1.

ORFs predicted from the RFHVMn genome sequence, with size and sequence homology to the corresponding ORFs from the KSHV and RRV genomes

ORF	Function or homolog	RFHV^a size (aa)	KSHV^b		RRV^c
ORF	Function or homolog	RFHV^a size (aa)	Size (aa)	% Identity^d	Size (aa)	% Identity
RF1	ITAM-containing signal transducing membrane protein; K1	528	289	22.6	423	16.1
4	Complement control protein; membrane protein; KCP	515	558	33.6	395	30.4
6	Single-stranded DNA binding protein (ssDBP); UL29	1,132	1,133	70.9	1,132	60.5
7	Subunit of terminase; UL28	688	695	56.9	686	51.3
8	Glycoprotein B; UL27	847	845	70.6	829	62.8
9	DNA polymerase; UL30	1,013	1,012	75.7	1,014	67.5
10	Derived from dUTPase; CMV UL82/83/84	411	418	44.0	416	30.1
-	(ORF 11; derived from dUTPase; CMV UL82/83/84)^f	-	407		409
RF2	vIL-6; K2	209	204	32.4	207	15.1
2	Dihydrofolate reductase (vDHFR)	208	210	54.1	188	40.7
RF3	E3 ubiquitin ligase membrane protein; K3; MIR1; ORF12	232	333	38.7	-
70	Thymidylate synthase (vTS)	344	337	64.6	333	61.6
RF4	Interleukin 8-like CC chemokine; K4; vMIP-II; vCCL-2	94	94	59.6	118	32.3
RF4.1	Interleukin 8-like CC chemokine; K4.1; vMIP-Ibeta; vCCL-3	102	114	48.2	-
RF4.2	Contains hydrophobic domains; K4.2	125	182	14.5	-
-	(E3 ubiquitin ligase membrane protein; K5; MIR2)^f	-	256		-
-	(Interleukin 8-like CC chemokine; vMIP-I; vCCL-1)^f	-	95		-
RF7	IAP-like inhibitor of apoptosis; K7; vIAP	171	126	17.3	-
16	Bcl-2-like inhibitor of apoptosis; vBcl-2	178	175	42.3	187	39.4
17	Minor capsid scaffold protein; UL26	546	553	47.7	536	41.1
17.5	Major capsid scaffold protein; UL26.5	300	288	35.0	291	32.2
18	Related to CMV UL79	255	257	66.1	257	54.9
19	Putative portal-capping protein; UL25	547	549	59.0	547	47.3
20	Nuclear protein; UL24	287	320	51.0	350	39.5
21	Thymidine kinase; UL23	564	580	55.5	557	38.2
22	Glycoprotein H; UL22	713	730	47.9	726	35.6
23	Tegument protein;UL21	411	404	53.1	402	46.1
24	Related to CMV UL87	752	752	64.1	732	54.4
25	Major capsid protein; UL19	1,378	1,376	82.1	1,378	74.5
26	Capsid triplex protein; UL18	305	305	78.0	305	63.3
27	Related to EBV BDLF2	293	290	41.7	269	28.0
28	Membrane protein	92	102	50.5	91	32.1
29	Putative ATPase subunit of terminase; UL15	685	687	72.1	690	52.7
30	Related to CMV UL91	96	77	62.3	76	31.7
31	Related to CMV UL92	202	224	64.9	217	46.3
32	Tegument protein; DNA packaging; UL17	451	454	44.0	464	36.3
33	Tegument protein; UL16	335	312	57.8	336	42.3
34	Related to CMV UL95	329	327	60.3	327	48.5
35	Tegument protein; UL14	150	151	56.0	149	32.0
36	Serine-threonine protein kinase; UL13	444	444	67.1	435	44.3
37	SOX; host shutoff; DNase; UL12	487	486	71.7	472	65.9
38	alkaline exonuclease; UL11	63	61	52.4	69	36.4
39	Glycoprotein M;UL10	393	400	64.3	378	60.3
40	Subunit of helicase-primase complex; UL8	642	669	38.2	654	31.2
42	Tegument protein; UL7	277	278	59.9	272	45.7
43	Portal protein; UL6	575	605	75.1	576	60.5
44	Helicase subunit of helicase-primase complex; UL5	782	788	74.4	790	66.0
45	Inhibition of IRF-7; virion phosphoprotein;	362	407	27.5	353	24.0
46	Uracil-DNA glycosylase; UL2	254	255	68.5	230	54.2
47	Glycoprotein gL; UL1	166	167	38.0	163	28.3
48	Unknown function; related to BRRF2 EBV	433	402	30.4	389	28.4
49	Unknown function; related to BRRF1 EBV	302	302	63.9	301	52.8
50	RTA; transactivator; related to BRLF1 EBV	665	631	48.3	577	36.7
RF8α	bZIP transcription factor; K8α	238	237	34.3	234	22.7
RF8.1	ORF51; K8.1; related to gp42 EBV	300	228	18.5	275	12.0
52	Related to BLRF2 EBV	133	131	51.9	139	44.6
53	Glycoprotein N; UL49A	104	108	43.5	104	42.3
54	Deoxyuridine triphosphatase; UL50	309	318	50.3	290	38.5
55	Tegument protein; UL51	222	227	66.7	210	51.1
56	Primase subunit of helicase-primase complex; UL52	838	843	57.8	828	50.5
57	Posttranscriptional regulator; UL54	462	455	53.5	442	42.0
RF9	vIRF-1; K9	466	449	35.6	415^e	16.9^e
RF10	vIRF-4; K10	773	911	20.3	-	-
RF10.5	vIRF-3; K10.5	601	566	22.0	-	-
RF11	vIRF-2; K11	424	467	27.5	-	-
58	Multiple transmembrane protein; UL43	358	357	51.8	360	38.2
59	DNA polymerase processivity factor; UL42	400	396	57.8	394	50.3
60	Ribonucleotide reductase small subunit; UL40	305	305	79.7	304	69.5
61	Ribonucleotide reductase large subunit; UL39	790	792	44.3	788	61.5
62	Capsid triplex protein; UL38	328	331	66.5	331	51.8
63	Tegument protein; UL37	926	928	44.5	939	36.8
64	Large tegument protein; UL36	2,604	2,635	45.8	2,548	16.2
65	Small capsid protein on hexon tips; UL35	187	170	42.8	169	38.3
66	BFRF2 EBV homolog; related to CMV UL49	427	429	57.3	448	43.4
67	Capsid docking protein on nuclear lamina; UL34	269	271	63.8	222	61.7
67A	DNA packaging; UL33	84	80	56.8	86	51.2
68	Nuclear localization of capsids and DNA packaging; UL32	462	467	60.2	457	47.6
69	Egress of capsids from nucleus; UL31	296	302	65.9	296	59.7
RF12	Related to Kaposin A; K12	212	60	19.0	-
RF13	FLIP-like inhibitor of apoptosis; vFLIP; K13	267	188	48.4	174	35.2
72	Cyclin D-like protein; vCyc	265	257	46.3	254	34.7
73	Latency-associated nuclear antigen; LANA	1,071	1,162	24.3	448	20.4
RF14	vOX2; contains two Ig domains; K14	300	314	33.3	253	32.0
74	vGPCR; G protein coupled receptor; similar to interleukin 8 receptor	336	342	56.4	342	43.1
75	Tegument protein; related to FGARAT	1,303	1,296	62.2	1,298	42.4
RF15	Signal transducing membrane protein; K15; LAMP	487	489	21.5	531	20.0

RFHV, RFHVMn genome (KF703446).

KSHV, KSHV genome (NC_009333).

RRV, RRV-26-95 (AF10726); KSHV-specific ORFs missing in RRV are indicated with hyphens.

Percent identity to the corresponding ORF from the RFHVMn genome.

Eightfold amplification of the RF9/K9 gene homolog—RRV ORF9-1 to ORF9-8.

KSHV-specific ORFs missing in RFHVMn are indicated in parentheses, and their relative positions within the RFHVMn genome are indicated by hyphens.