Table 1.

Averaged parameters of PLS models^* of NIR spectra for prediction of absolute, percent and free water content in hyaline cartilage.

	PLS Parameter	Absolute water (mg)	Percent water	Percent water (Physiological region)	Percent free water
A	R2 of test model (calibration)	0.86±0.008	0.92	0.89±0.009	0.93±0.004
B	RMSE	2.8±0.094	5.80±0.012	2.6±0.130	2.45±0.0326
C	R2 of validation	0.84±0.012	0.92±0.0003	0.88±0.0007	0.94±0.002
D	Correlation Coefficient	0.92±0.004	0.96±0.0002	0.94±0.003	0.97±0.001
E	Factors	7	1	7	4
F	RMSEP	3.05 ±0.113mg	5.81±0.012%	2.68±0.125	2.51±0.023%

^*MSC-corrected second derivative spectra (420) were used in each model, and data is presented for n = 3 models, ± standard deviation. Parameters A to E were calculated from the cross-validation models. Root mean square (RMSE) of validation was measured to determine the overall error of the model. Parameter F, the root mean square error of prediction (RMSEP), was calculated from the independent prediction of the remaining 252 spectra.