Table 4. Peptides identified by ion-trap mass spectrometry analysis of the 26 kDa degradation band: 8-mer, 15-mer and 18-mer; and their alignment with the original sequence from the proteins ω- and γ-gliadin and LMW glutenin from which they proceeded.
| Peptide | Prolamin | Sequence alignment |
|---|---|---|
| 8-mer | FPLQPQQP | |
| ω-gliadin | -S.FPLQPQQP.F- | |
| 15-mer | PFIQPSLQQQLNPCK | |
| γ-gliadin | -P.PFIQPSLQQQLNPCK.N- | |
| 18-mer | VFLQQQCSPVAMPQSLAR | |
| LMW-glutenin | -K.VFLQQQCSPVAMPQSLAR.S- |
In bold, cleaving points that could not had been generated through the technical process involving trypsin digestion and therefore likely to be derived from biological protease activities.