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. Author manuscript; available in PMC: 2013 Nov 22.
Published in final edited form as: J Endocrinol. 2006 Oct;191(1):159–170. doi: 10.1677/joe.1.06557

Figure 6.

Figure 6

Effect of essential and non-essential amino acids (NEAAs) on GLP-1 secretion. (A) Cells were incubated for 2 h with increasing concentrations of a mixture of essential amino acids (EAA; MEM, Sigma no. 5550) or a mixture of NEAAs (Sigma no. 7145; n=3). The concentrations displayed for both EAA and NEAA equate to 1=0·69 g/l, 2=3·47 g/l and 3=6·90 g/l. *EAA 3 is significantly different from all other treatments (P<0·05). (B) Cells were incubated for 2 h with equal concentrations (matched for nitrogen content) of MH, EAA or NEAA or two control solutions differing in pH alone (n=3). *Secretion of GLP-1 with MH and EAA was significantly greater than control or NEAA treatments (P<0·05). No effect of pH was seen on GLP-1 secretion.