Abundance of p38 following incubation with essential and NEAAs. Western blots were performed with the phosphospecific and non-phosphorylated form of p38 antibodies (n=3). Ratios of phosphorylated:non-phosphorylated p38 signals were calculated and normalized to control. (A) *Significant activation of p38 following incubation with EAA at 2, 5, 15 and 60 min (P<0·05). (B) Representative Western blots shown with lane assignments as follows: C, control; 2, 2 min; 5, 5 min; 15, 15 min; 60, 60 min; MH, 2% MH at 5 min.