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. 2013 Nov 22;8(11):e79800. doi: 10.1371/journal.pone.0079800

Figure 4. Progress curves of the manno-oligosaccharides generated by the wild-type PaMan5A and the PaMan5A-K139R/Y223H variant upon hydrolysis of mannohexaose.

Figure 4

18.2 nM of the wild-type PaMan5A (A) and the PaMan5A-K139R/Y223H variant (B) were incubated with 1 mM of mannohexaose in acetate buffer pH 5.2 at 40°C. The amount of each manno-oligosaccharide, i.e., mannobiose (full circles), mannotriose (full squares), mannotetraose (crosses), and mannohexaose (full diamonds), is indicated during the course of the reaction.