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. 2013 Nov 22;8(11):e80643. doi: 10.1371/journal.pone.0080643

Figure 3. Southern blot of genomic DNA isolated from wild-type (lane 1-2) and transgenic (lane 3-10) plants using a digoxigenin-labeled NPTII probe.

Figure 3

Lane 1: wild-type plant digested with HindIII; lane 2: wild-type plant digested with EcoRI; lane 3: transgenic plant carrying the pECS-GUS fusion digested with HindIII: lane 4: transgenic plant carrying the pECS-GUS fusion digested with EcoRI; lane 5: transgenic plant carrying the pFS-GUS fusion digested with HindIII: lane 6: transgenic plant carrying the pFS-GUS fusion digested with EcoRI; lane 7: transgenic plant carrying the pCPS-GUS fusion digested with HindIII: lane 8: transgenic plant carrying the pCPS-GUS fusion digested with EcoRI; lane 9: transgenic plant carrying the pADS-GUS fusion digested with HindIII: lane 10: transgenic plant carrying the pFS-GUS fusion digested with EcoRI; lane 11: positive control (882 bp fragment carrying the NPTII gene). Sample size: 10–15 µg/lane.