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. 2013 Nov 22;8(11):e82295. doi: 10.1371/journal.pone.0082295

Figure 2. Localization of transgene expression from the human EF1 promoter in PEGPOD pcpg-LacZ.

Figure 2

(A) Flatmounts of RPE/Choroid/Sclera from Balb/C mice injected in the subretinal space with PEGPOD pcpg-LacZ or PEGPOD pcpg-GDNF NPs assayed for LacZ activity 48 hours post injection. PEGPOD pcpg-LacZ injected eyes, but not PEGPOD pcpg-GDNF injected eyes, show strong LacZ activity throughout the eyecup. (B) Transverse sections through the retina of eyes injected with PEGPOD pcpg-LacZ show LacZ expression along the RPE with no expression in the retina. No LacZ activity was observed in retinal sections of eyes injected with control NPs. RPE, retinal pigment epithelium; ONL, outer nuclear layer; GCL, ganglion cell layer.