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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Transgenic Res. 2013 Jul 16;22(6):10.1007/s11248-013-9729-0. doi: 10.1007/s11248-013-9729-0

Fig.4. Identification of pB transposon integration sites in transgenic pig’s genome.

Fig.4

Chromosomal DNA sequences flanking the inserted pB transposon were identified by sequencing the inverse PCR products. The obtained flanking DNA sequences were then used to blast against Sus scrofa (pig) genomic DNA database to find out their location in pig genome. All identified insertion site sequences contain the TTAA sequences which is indicative of the insertion border between the pB transposon and flanking genomic DNA. (A). An identified integration site of pB transposon on chromosome 4. The pB transposon was inserted in between 716 bp and 717 bp of GenBank sequence (GenBank number NW_003534687.2, length=657315 bp). (B). An identified integration site of pB transposon on chromosome 2. The pB transposon was inserted in between 96558 bp and 96559 bp of GenBank sequence (GenBank number NW_003299559.3, length=172486 bp).