Table 1.
Summary of primers
| Name | Use | Sequence |
|---|---|---|
| 5Sma1163-1166 | Mapping the Nia polycistron | AGGTCCACAGCCTCACCTCC |
| 3Sma1163-1166 | Mapping the Nia polycistron | CCTGCACCGTGGATGGACAG |
| 5Sma1166-1168 | Mapping the Nia polycistron | AGCCATATTCGCCGCCAGGAGCA |
| 3Sma1166-1168 | Mapping the Nia polycistron | TCCTCTCGGGCCTGCTCCAGG |
| Sma1163 qF | qPCR of Nia | GACCTCCCGGAAGACGAGGT |
| Sma1163 qR | qPCR of Nia | CTCATCCCGGCAAAAGCATC |
| 16S-F | qPCR of 16S rRNA | GATAAGCCGAGAGGAAGGTG |
| 16S-R | qPCR of 16S rRNA | GTGTAGCCCAGCCCGTAAG |
| 5Sma1163 | Cloning of Nia and ΔHr-Nia in pBAD-TOPO |
ATGCGGGATATTGCGCAGA |
| 3Sma1163 | Cloning of Nia in pBAD- TOPO |
CTCGTGCTCGAGCGATCGAT |
| 3ΔHrSma1163 | Cloning of ΔHr-Nia in pBAD- TOPO |
CGCATGCTCCAAACGTGTTGTTTT |
| 5Nia-Hr | Cloning of Nia-Hr in pPR- IBA1 |
ATGGTAGGTCTCAAATGGGCGATCCCATGCG CGGCTG |
| 3Nia-Hr | Cloning of Nia-Hr in pPR- IBA1 |
ATGGTAGGTCTCAGCGCTCTCGTGCTCGAGC GATCGATAG |