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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Clin Exp Pharmacol Physiol. 2013 Dec;40(12):10.1111/1440-1681.12151. doi: 10.1111/1440-1681.12151

Fig 2.

Fig 2

Effect of KCNJ5 and mutated KCNJ5-T158A lentiviral transduction on steroid synthesis in the HAC15 cell. The upper panel shows the effect of transduction of HAC15 cells with wild type KCNJ5 lentivirus on aldosterone and cortisol secretion in cells under basal conditions and stimulated with angiotensin II (10 nM) and forskolin (10µM) for 24 hrs (*p<0.05, **p<0.01). Transduction of cells resulted in a decrease in basal and stimulated aldosterone and cortisol secretion (data redrawn from Oki et al by permission 31.

The lower panel shows the effect of transduction of HAC15 with a lentivirus with the mutated KCNJ5-T158A on aldosterone, cortisol and 18-oxocortisol secretion under basal and stimulation with angiotensin II (10nM) and forskolin (10 µM). There was a significant enhancement of steroid secretion in the transduced cells with the KCNJ5-T158 lentivirus (data redrawn from Oki et al by permission) 34.