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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Eur J Immunol. 2013 Aug 12;43(11):10.1002/eji.201243068. doi: 10.1002/eji.201243068

Figure 4. FCRL3 inhibits CpG-induced PC differentiation and Ig production.

Figure 4

(A) Purified CB B cells were treated and cultured as in Fig. 3. Cells were harvested on day 4 for staining with mAbs specific to CD19, CD27, IgM, IgG, and CD38. Gates within the contour plots encompass CD38++CD27++, IgM+CD27++, and IgG+CD27++ PCs. Values indicate the total number and percentage of cells from all events harvested from single wells of a representative panel and (B) summarizes data enumerated in triplicate. (C) CB B cells treated as in panel A were analyzed by ELISPOT. On day four of culture, cells were plated in light-chain reactive antibody coated wells and incubated overnight prior to PC quantitation. (D) Ig production in supernatants harvested on day 4 was assessed by capture ELISA. The data are mean ± SD of triplicate cultures and are representative of experiments from three different donors. ** P < 0.001 vs CpG plus the IgG1 control by two-tailed t-test.