Skip to main content
. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Aging Cell. 2013 Aug 11;12(6):10.1111/acel.12135. doi: 10.1111/acel.12135

Fig. 1. Inverse correlation between miR-34a and NAD+ levels.

Fig. 1

(A-C) HF diet-induced obese mouse experiments. (A) Hepatic miR-34a levels in lean and dietary obese mice. (B) Hepatic NAMPT/SIRT1 protein levels in lean and diet-induced obese mice were detected and the ratio to laminin is plotted in the right panel. (C) Hepatic NAD+ and NADH levels are shown. (D-F) Overexpression of miR-34a. Primary mouse hepatocytes were infected with Ad-viral vectors as indicated and miR-34a levels (D), NAMPT/SIRT1 protein levels (E) and NAD+ and NADH levels (F) were measured. (G-I) Downregulation of miR-34a. Conversely, hepatocytes were transfected with anti-miR-34a and miR-34a levels (G), NAMPT protein levels (H), and the NAD+ and NADH levels (I) were measured. The mean and SEM are plotted (n=4), *, p<0.05, **, p<0.01.