Fig 1.

Flow cytometry gating strategy to detect cytokine production and expression of maturation factors by DC in WB. [A] Among viable cells, DC are identified as negative for the lineage markers (Lin-1) and positive for HLA-DR [B] HLA-DR^pos and Lineage−1^neg DC are then phenotyped as myeloid (CD123^low, CD11c^high) or plasmacytoid (CD11c^low, CD123^high) DC. [C] Expression of maturation factors (CD83, CD80 and CCR7) and cytokines (TNF-α and IFN-α) on DC subsets after TLR7/8 stimulation including isotype and unstimulated controls.