Fig. 3.

DENV2 infection induced ROCK activation and phosphorylation of vimentin at Ser71. At various time points after DENV2 infection, cell lysates were analyzed by ELISA and Western blotting. a ROCK levels were measured by ELISA. A histogram depicts the change in ROCK activation. Each reaction was performed in duplicate, and each bar represents the mean + the standard deviation for three experiments. *P < 0.05. b Western blot analysis was performed to detect Ser71-phosphorylated vimentin and vimentin expression, respectively. The results show that DENV2 induced an increase in the phosphorylation of vimentin at Ser71 from 15 min to 24 h, with a maximum increase of 7.8-fold above control cells at 8 h p.i. Each blot represents data from a minimum of six separate experiments