Table 2.
Acrosomal integrity (%) of Sprague–Dawley epididymal rat sperm stored at 4, 22, and 37 °C for 24, 48, and 72 h
| Temperature | Extender | After dilution | 24 h | 48 h | 72 h |
| 4 °C | TL-HEPES | 91.8 ± 2.7 | 74.7 ± 4.6 | 71.8 ± 2.6 | not done |
| Tris | 91.8 ± 1.9 | 69.7 ± 5.6 | not done | not done | |
| mKRB | 93.3 ± 2.2 | not done | not done | not done | |
| LEY | 91.8 ± 3.5 | 77.2 ± 4.3 | 73.7 ± 3.4 | not done | |
| PBS | 90.2 ± 2.2 | 64.0 ± 6.6 | not done | not done | |
| 22 °C | TL-HEPES | 91.8 ± 2.7 | 78.7 ± 6.1 | 67.5 ± 6.6a | 62.2 ± 7.6a |
| Tris | 91.8 ± 1.9 | 77.7 ± 4.4 | 75.7 ± 1.9a | not done | |
| mKRB | 93.3 ± 2.2 | 74.0 ± 5.6 | 71.8 ± 3.9a | 67.8 ± 3.7a | |
| LEY | 91.8 ± 3.5 | 75.7 ± 5.4 | 54.5 ± 7.9a | 37.0 ± 2.8a | |
| PBS | 90.2 ± 2.2 | 75.5 ± 5.8 | 67.7 ± 9.0a | 57.3 ± 8.9a | |
| 37 °C | TL-HEPES | 91.8 ± 2.7 | 74.0 ± 2.6 | not done | not done |
| Tris | 91.8 ± 1.9 | 71.2 ± 3.6 | not done | not done | |
| mKRB | 93.3 ± 2.2 | 72.0 ± 1.9 | not done | not done | |
| LEY | 91.8 ± 3.5 | not done | not done | not done | |
| PBS | 90.2 ± 2.2 | 78.2 ± 3.7 | not done | not done |
Data are presented as mean ± SEM (n = 6).
a
Values within a temperature and duration are significantly (P < 0.05) different.