Table 1.
Intra- and Inter-Day Variation in Liraglutide Protein Binding
| Intra-Day | Mean (SD) Liraglutide Bound [%] | Inter-Day | Mean (SD) Liraglutide Bound [%] |
|---|---|---|---|
| Run 1 | 99.9 (0.03) | Day 1 | 99.9 (0.03) |
| Run 2 | 99.7 (0.35) | Day 2 | 99.8 (0.04) |
| Run 3 | 99.8 (0.06) | Day 3 | 99.8 (0.04) |
| Run 4 | 99.9 (0.03) | Day 4 | 99.8 (0.02) |
| Run 5 | 99.7 (0.22) | Day 5 | 99.6 (0.19) |
| Run 6 | 99.8 (0.05) | ||
| Overall mean (SD) | 99.8 (0.10) | 99.8 (0.11) | |
| Coefficient of variation (%) | 0.10 | 0.11 |
Data are unadjusted means (SD).
Liraglutide concentration of the plasma incubation solution was 104 pM, and the inner–outer liraglutide ratio was 100:1.
The intra-day evaluation involved repetition of the assay six times in 1 day, whereas the inter-day assay involved repetition of the assay on 5 separate days.