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(A) Replacement of the endogenous ecmA gene with GFP (B) prestalk specific expression of GFP knock-in strain at slug and culminant stages. (C) FACS analysis of Ax3 wild type and gefE− mutant GFP knock-in strains stimulated with 0.01–100 nM DIF for 9 hr. Y-axis shows the number responders and X-axis the GFP expression level per cell. (D) Median GFP expression level of wild type and gefE− mutant populations stimulated with 0.01–100 nM DIF.
